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The delicate-clipped sequences that overhang the breakpoint are collapsed into a consensus sequence utilizing a multiple sequence alignment (MSA) delivered inside the seqAn library [27]. Wham applies a few filters into the consensus sequences. Breakpoints aren't described in scenarios where consensus sequences are shorter than 10 bp or comprise a l
Wham is made for paired-conclude Illumina libraries with standard insert measurements (~300bp-500bp). Wham integrates mate-pair mapping, split examine mapping, gentle-clipping, choice alignment and consensus sequence centered evidence to predict SV breakpoints with solitary-nucleotide precision. Wham generates a put together pileup (catalog of read